表面修饰的聚对苯二甲酸乙二醇酯倾斜纳米线板对细胞生长的影响

Effect of surface-modified PET nanowire plates on cell growth

摘要:

目的 纳米材料在生物医学领域的应用上有独特优势，多项研究表明材料的物理性质会影响到细胞的生长特性。该研究基于高分子聚对苯二甲酸乙二醇酯（PET）材料，在PET表面进行纳米线角度修饰，以观察基质表面物理特性改变对细胞生长的影响。方法 将张氏肝细胞分别培养在玻璃、聚苯乙烯（PS）和表面进行了不同倾斜角度修饰的PET材质上，24 h后收集不同基质上的细胞，碘化丙啶（PI）染色后用流式细胞仪检测细胞周期。采用MTT法检测细胞存活率，鬼笔环肽（phalloidin）和DAPI染色制作细胞爬片以观察细胞形态。结果 发现各组细胞的生长周期分布无明显差别，PET纳米线板生长的细胞存活率与玻璃板的比较差异无统计学意义（P>0.05），但此两者上的细胞存活率比在PS基质生长的低（P<0.05），而倾斜角为40°的PET纳米线板的细胞存活率与其他不同倾斜角度PET纳米线板比较差异有统计学意义（P<0.05）。观察细胞形态发现生长于10°、20°的PET纳米线板上的细胞与其他角度的纳米线板和玻璃板相比表现出一定的生长方向性。结论 该研究证实了采用PET表面修饰的纳米线板作为细胞生长基质的可能性，支持作用与传统玻璃材质类似，其表面进行角度修饰后，表现出引导细胞生长方向的作用，在组织修复支撑等方面可能存在潜在的应用价值。

Abstract:

Objective Nanomaterials have unique advantages in their applications in the biomedical field, many studies have shown that the physical properties of materials could affect the growth characteristics of cells. This study is based on the polymer polyethylene terephthalate (PET) material, the nanowire angles were modified on the PET surface to observe the impact of changing the physical properties of the matrix surface on cell growth.Methods Zhang's hepatocytes were cultured on glass, polystyrene (PS) and PET materials with different surface tilt angles. After 24-hour growth the cells were collected from different substrates, and the cell cycle was detected by flow cytometry using propidium iodide (PI) staining. Using the thiazole blue (MTT) method to detect the cell survival rate, phalloidin and DAPI staining was used to prepare cell slides for observing cell morphology.Results We found that there was no significant difference in the growth cycle distribution of the cells in each group and the cell survival rate between PET and glass had no significant difference (P>0.05), but the cell survival rate on both plates was lower than that on PS matrix (P<0.05), and there was significant difference in cell survival rate between the PET nanowire plate with a tilt angle of 40° and other PET nanowire plates with different tilt angles (P<0.05). Observation of cell morphology revealed that cells grown on PET nanowire plates 10°, 20°showed a certain growth direction compared with nanowire plates and glass plates at other angles.Conclusion This study confirmed the possibility that the nanowire plate modified with PET surface can be used as a cell growth matrix, the support function is similar to the traditional glass material, after angle modification on its surface, it exhibits a guiding effect on cell growth direction and may have potential application value in tissue repair and support.