N-MID、Ghrelin、miR-133a在创伤性骨折中的表达及与延迟愈合的相关性研究

Expression of N-MID, Ghrelin, and miR-133a in traumatic fractures and their correlation with delayed healing

摘要:

目的 探究在创伤性骨折中血清骨钙素N端中分子片段（N-MID）、饥饿素（Ghrelin）、微小RNA-133a（miR-133a）的表达以及与延迟愈合的相关性。方法 选取2022年6月至2023年6月南阳市中医院独山院区收治的60例患有创伤性骨折延迟愈合的患者作为研究组，另选取60例正常愈合的患者作为对照组。比较两组患者血清N-MID、Ghrelin以及miR-133a水平，分析三者与延迟愈合的相关性。结果 研究组N-MID、Ghrelin水平低于对照组，且miR-133a水平高于对照组（P<0.05）；Ⅲ期、Ⅳ期患者N-MID、Ghrelin水平低于Ⅱ期患者，且miR-133a高于Ⅱ期患者（P<0.05）；Ⅳ期患者N-MID、Ghrelin水平低于Ⅲ期患者，且miR-133a高于Ⅲ期患者（P<0.05）；创伤性骨折延迟愈合患者N-MID、Ghrelin水平呈正相关（r=9.409, P=0.003）；N-MID、miR-133a水平呈负相关（r=-6.442, P=0.013）；Ghrelin、miR-133a水平呈负相关（r=-5.677, P=0.019），N-MID、Ghrelin与创伤性骨折延迟愈合呈正相关，miR-133a与创伤性骨折延迟愈合呈负相关。结论 N-MID、Ghrelin、miR-133a水平都与创伤性骨折的愈合程度有关，创伤性骨折患者中miR-133a过度表达抑制N-MID、Ghrelin水平，从而影响骨折的正常愈合，检测血清N-MID能评价创伤性骨折的恢复情况，Ghrelin可作为预测创伤性骨折延迟愈合的潜在标志物。

关键词:血清骨钙素N端中分子片段;饥饿素;微小RNA-133a;创伤性骨折

Abstract:

Objective To investigate the expression of N-MID, Ghrelin, and miR-133a in traumatic fractures and their correlation with delayed healing.Methods Sixty patients with delayed healing of traumatic fractures admitted to Dushan Campus of Nanyang Traditional Chinese Medicine Hospital from June 2022 to June 2023 were selected as the study subjects, and another 60 patients with normal healing were selected as the control group. The levels of N-MID, Ghrelin, and miR-133a in the serum of two groups of patients were compared, and their correlation with delayed healing was analyzed.Results The levels of N-MID and Ghrelin in the study group were lower than those in the control group, and the levels of miR-133a were higher than those in the control group (P<0.05). The levels of N-MID and Ghrelin in stage III and IV patients were lower than those in stage Ⅱ patients, and miR-133a was higher than that in stage Ⅱ patients (P<0.05). The levels of N-MID and Ghrelin in stage Ⅳ patients were lower than those in stage Ⅲ patients, and miR-133a was higher than that in stage Ⅲ patients (P<0.05). The levels of N-MID and Ghrelin in patients with traumatic fractures were positively correlated (r=9.409, P=0.003). The levels of N-MID and miR-133a were negatively correlated (r=-6.442, P=0.013). The levels of Ghrelin and miR-133a were negatively correlated (r=-5.677, P=0.019). N-MID and Ghrelin are positively correlated with delayed healing of traumatic fractures, while miR-133a is negatively correlated with delayed healing of traumatic fractures.Conclusion The levels of N-MID, Ghrelin, and miR-133a are all related to the degree of healing of traumatic fractures. Overexpression of miR-133a inhibits the levels of N-MID and Ghrelin in patients with traumatic fractures, thereby affecting the normal healing of fractures. Detecting serum N-MID can evaluate the recovery of traumatic fractures, and Ghrelin can serve as a potential marker for predicting delayed healing of traumatic fractures.

Key words:N-terminal intermediate molecular fragments of serum osteocalcin;Ghrelin;micro RNA-133a;traumatic fracture