生物信息学分析髓系细胞触发受体-2对低级别胶质瘤的影响

Bioinformatics analysis of the effect of triggering receptor expresses on myeloid cells-2 on low-grade glioma

摘要:

目的 通过生物信息学手段探究髓系细胞触发受体-2（TREM-2）在低级别胶质瘤（LGG）中的表达情况及对患者生存率的影响，为LGG的早期发现、早期诊断及治疗提供潜在的靶点。方法 运用在线网站GEPIA2和UALCAN分析TREM-2在LGG肿瘤组织和正常组织中的表达差异；与年龄、性别、种族、肿瘤分级等临床病理特征之间的关系；以及TREM-2的LGG肿瘤组织中的异常表达对LGG患者总体生存率的影响。DAVID 6.8软件对TREM-2相关基因进行KEGG通路富集分析；使用TIMER数据库分析TREM-2表达与肿瘤纯度和免疫细胞浸润程度之间的相关性。结果 与正常组织相比，TREM-2在LGG肿瘤组织中的表达较正常组明显升高（P<0.05），并且与肿瘤分级和组织学分型相关（P<0.001），而与患者的年龄、性别及种族不相关（P>0.05），生存分析表明TREM-2与LGG预后密切相关，LGG表达越高，患者的总体生存率越低（P=0.00025和P=0.0029），同时无病生存率也降低（P=0.015）；通路富集结果表明与TREM-2正相关基因通路主要涉及结核、癌症通路、吞噬体等，负相关基因通路主要涉及MAPK信号通路、神经活性配体-受体相互作用等；TREM-2的表达与肿瘤微环境中6种免疫细胞的浸润水平呈正相关。结论 TREM-2在LGG肿瘤组织中表达明显升高，并且与患者生存率相关，是LGG诊断和治疗的潜在靶点。

Abstract:

Objective To explore the expression of triggering receptor expresses on myeloid cells-2 (TREM-2) in low-grade glioma (LGG) and its impact on patient survival by bioinformatics, and to provide potential targets for the early detection, early diagnosis and treatment of LGG.Methods The online websites GEPIA2 and UALCAN were used to analyze the expression difference of TREM-2 in LGG tumor tissue and normal tissue, the relationship with clinicopathological characteristics such as age, gender, race, tumor grade, and the effects of abnormal expression of TREM-2 in LGG tumor tissue on the overall survival rate in LGG patients. KEGG pathway enrichment analysis of TREM-2-related genes was conducted by DAVID 6.8 software. The correlation between TREM-2 expression and tumor purity and degree of immune cell infiltration was analyzed using the TIMER database.Results Compared with normal tissues, the expression of TREM-2 in LGG tumor tissues was significantly higher than that in the normal group (P<0.5), and it was correlated with tumor grade and histological type (P<0.001) and not correlated with the patient's age, gender and race (P>0.05). Survival analysis showed that TREM-2 was closely related to the prognosis of LGG. The higher the expression of LGG, the lower the overall survival rate of patients (P=0.00025 and P=0.0029), and disease free survival rate also decreased (P=0.015). Pathway enrichment results showed that the gene pathways positively related to TREM-2 mainly involved tuberculosis, cancer pathway, phagosome, etc., and the negatively related gene pathways mainly involved MAPK signaling pathway, neuroactive ligand-receptor. The expression of TREM-2 was positively correlated with the infiltration level of 6 kinds of immune cells in the tumor microenvironment.Conclusion The expression of TREM-2 in LGG tumor tissues is significantly increased, and is related to the survival rate of patients. It is a potential target for the diagnosis and treatment of LGG.