HR-HPV感染患者微量元素与阴道微环境中细胞因子的相关性研究

Correlation between serum trace elements and cytokines in vaginal microecology in patients with HR-HPV infection

摘要:

目的探究高危型人乳头瘤病毒(HR-HPV)感染患者的血清微量元素与阴道微环境中细胞因子的相关性。方法选取2017年1月至2019年5月新乡市中心医院收治的66例感染HR-HPV宫颈病变患者,根据临床诊断分为宫颈上皮内瘤(CIN)Ⅰ组20例[平均年龄(43.02±8.43)岁]、CINⅡ~Ⅲ组25例[平均年龄(42.17±9.62)岁]及宫颈癌组21例[平均年龄(45.17±8.89)岁]。择取同期该院体检的20例健康女性作为对照组[平均年龄(47.33±7.81)岁]。采用酶联免疫吸附法检测阴道微环境中γ干扰素(IFN-γ)、肿瘤坏死因子-α(TNF-α)、白细胞介素-10(IL-10)、转化生长因子-β(TGF-β)水平。采用电子耦合-等离子体原子发射光谱仪检测血清中锌、硒、铁、锰、钴等微量元素。结果①对照组血清中锌、硒水平>CINⅠ组>CINⅡ~Ⅲ组>宫颈癌组(P<0.05)。②4组阴道微环境中细胞因子IFN-γ、TGF-β、IL-10、TNF-α水平比较,差异有统计学意义(P<0.05)。③血清锌含量与阴道中IFN-γ、TNF-α水平呈正相关(r=0.573和0.612,P=0.007和0.009);而与阴道中IL-10、TGF-β水平呈负相关(r=-0.193和-0.416,P=0.002和0.003)。结论HR-HPV感染患者血清微量元素锌显著降低,其与疾病发生、发展及病程中免疫反应密切相关。

Abstract:

【Objective】To examine the correlation between serum trace elements and cytokines in vaginal microecology in patients with HR-HPV infection.【Methods】Patients with cervical intraepithelial neoplasms(CIN),Ⅰstage cervical cancer and health women were enrolled in Xinxiang Central Hospital.According to the results of clinical diagnosis,they were divided into four groups:CINⅠgroup(20 cases),CINⅡ-Ⅲgroup(25 cases),cervical cancer(21 cases),control group(20 cases).IFN-γ,TNF-α,IL-10,TGF-βin vaginal microecology were detected by ELISA,and the content of Zn,Se,Fe,Mn,Co were determined by ICPAES.【Results】Serum Zn and Se levels decreased significantly in the order control>CINⅠ>CINⅡ+Ⅲ>cancer,there was a statistical difference in comparison(P<0.05).No significant difference in Fe,Mn and Co were found among four groups(P>0.05).The levels of IFN-γand TNF-αin vaginal microecology decreased significantly in the order control>CINⅠ>CINⅡ+Ⅲ>>cancer;while IL-10 and TGF-βlevels increased significantly in the order control