Objective: To evaluate the problems in the nosocomial infection management and control effect by the investigation of nosocomial infection (NI) prevalence. Methods: By combination of bed-side and medical records survey,questionnaires about NI cases in one day were filled and analysed. Results: 1141 patients were investigated, NI prevalence rate was 6.4%, case infection rate was 6.75%; the most common infection site was surgical site, followed by lower respiratory tract, upper respiratory tract and gastrointestinal tract. Conclusion: It should be paid attention to the rational use of antimicrobial agents,the management of key units should be strengthened,prospective surveillance should be stressed to prevent and control nosocomial infection.