提取大白鼠脂肪干细胞的初步研究

Stem cell from murine adipose tissue

摘要:

目的探讨自脂肪中分离干细胞的方法.方法取大白鼠皮下脂肪组织,经消化、离心、去除红细胞等步骤,培养,间日换液.结果出现呈梭形成纤维细胞样生长的细胞,细胞增殖旺盛.结论可自大鼠皮下脂肪获得多能干细胞.

Abstract:

Objective: To discuss the method of isolating stem cell from adipose tissue. Methods: The subcutaneous adipose tissue from murine was digested, centrifugated, deodrythrocyted, etc and cultivated with DMEM/F12. The cultivation was exchanged once 2 days. Results: The cell spread fusiform shape, and proliferation with rapid rate. Conclusion: The cell from murine subcutaneous adipose tissue may be multipotential stem cell.