抑癌基因p16突变与鼻咽癌的相关研究

Study on p16 gene and its mutation in nasopharyngeal carcinoma

摘要:

目的研究鼻咽癌p16基因的状态.方法采用多重PCR分析法,单链构像多态性分析法(SSCP)对48例鼻咽癌标本与相应的癌旁组织中p16基因突变情况进行了检测.结果48例鼻咽癌标本中16例p16基因第2外显子缺失,相应的癌旁组织未检出.结论p16基因在鼻咽癌中存在高频率缺失,其改变与鼻咽癌发生发展有密切关系.

Abstract:

Objective: To investigate the status of p16 gene in nasopharyngeal carcinoma (NPC). Methods: Total of 48 NPC cases and correspondent tumor-adjacent were detected for p16 gene munations by using multiplex polymerase chain reaction (PCR) and single strand conformation polymorphism (SSCP) respectively. Results: Among 48 NPC cases, 16 cases homozygous deletions and mutation were not identified. However, deletion and mutation were not found in all the tumor-adjacent tissues. Conclusions: The data above shows that p16 gene loss in high frequency in nasopharyngeal carcinoma. The alteration of p16 gene may play an important role in nasopharyngeal carcinoma.