Abstract:Objective: The aim of this study was to observe the hepatic targeting of Gal-HSA magnetic nanoparticles containing adriamycin and its effect of magnetic field application to targeting distribution in vivo,and probe into distributed characterization in the normal rat organs. Methods: Abominal exposure was carried out through a midline abdominal incision. A cannula was inserted into the hepatic artery and fixed up it. Gal-HSA magnetic nanoparticles containing adriamycin were injected into hepatic artery (equivalent dose of free adriamycin 0.5 mg/kg), after the left lateral lobe of the liver was expose to the magnetic field for 30 minute. The rat was immediately killed. Administration of equal close of nanoparticles in the absence of magnetic field served as a control. The targeting liver tissue, non-targeting liver, kindey, heart, lung, small intestine, spleen and blood was analyzed for 125I-counts. Liver tissue was examined for histology. Results: In either experimental group or control group, the radioactivity of heart kidney, lung, spleen small intestine was much less than liver (included target site and non-targeting site). The radioactivity of lung in experimental group decreased significantly, relative to dose distributed in liver (P<0.01). In addition, 75~85% of injected radioactivity dose distributed in liver. The histology examination demonstrated a lot of nanoparticles accumulated in arteriola.in control group, no accumulation of nanoparticles was found, only some single nanoparticles dispersed in hepatic sinus.Conclusions: Gal-HSA magnetic nanoparticles containing adriamycin in normal rat liver had remarkable hepatic targeting and good magnetic targeting. Dose which Gal-HSA magnetic nanoparticles containing adriamycin injected mainly distributed in liver, the ratio of heart, kidney, spleen,small intestine and blood in experiment group descreased significantly compared to control. It means the relative exposure of these organs in experimental group reduced, compared to control.
