Objective: To explore the medical ethical problem in the seeding cells of tissue engineering. Methods: To discuss the ethical problem and countermeasure from the source of the seeding cells. Results: Patient's rights to know the facts of test, efficacy and security of clinical application must be fully ensured during implantation of seeding cells under the laws. Conclusions: To consummate related standard of tissue engineering, and to search for the source which widely exists and is easily obtained as soon as possible.