生长因子(EGF和PDGF-BB)对小鼠骨髓基质干细胞增殖的作用及其意义

Effects and significance of EGF and PDGF-BB on proliferation of murine bone marrow stromal stem cells in vitro

摘要:

目的探讨表皮生长因子(epidermal growth factor,EGF)和血小板源生长因子(platet-derived growth factor,PDGF-BB)对体外培养的小鼠骨髓基质干细胞(murine bone marrow stromal stem cells,mMSCs)增殖的作用,探索小鼠骨髓基质干细胞体外快速增殖的条件和方法.方法将小鼠骨髓基质干细胞进行分离和纯化培养后,加入EGF和PDGF-BB,利用3H胸腺嘧啶核苷(3H-TdR)掺入反映细胞增殖效果,倒置显微镜下观察细胞增殖及分化状态.结果EGF和PDGF-BB干预后,3H-TdR掺入(11456.14±968.86)dpm较对照组(3271.88±420.91)dpm明显增高,说明EGF和PDGF-BB可促进小鼠骨髓基质干细胞的增殖;倒置显微镜下观察可见EGF和PDGF-BB处理组mMSCs具有增殖优势,分化相对延迟,而对照组提前显示出自然分化现象.结论生长因子(EGF和PDGF-BB)可促进小鼠骨髓基质干细胞的增殖,是小鼠骨髓基质干细胞体外大量培养和快速增殖的有效刺激因子.

Abstract:

Objective: To investigate the effects of EGF, PDGF-BB on the proliferation in cultured murine bone marrow stromal stem cells (mMSCs) and to find a well-proliferation condition and method of murine bone marrow stromal stem cells in vitro. Methods: Murine bone marrow stromal stem cells were isolated and cultured in vitro before EGF and PDGF-BB were added in. Proliferation was measured with 3H-Thymidine incorporation. Results: EGF (10 ng/ml) and PDGF-BB (10 ng/ml) cause increases of 3H-TdR incorporation (l1456.14+968.86)dpm more than the control group (3271.88±420.91)dpm signifieatively(P＜0.05).Conclusions: EGF and PDGF-BB were effective and available for mMSCs proliferation in vitro.